File Name: isolation and screening of industrially important microorganisms .zip
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The search for novel bioactive compounds from the natural environment has rapidly been gaining momentum with the increase in multi-drug resistant MDR pathogens. In the present study, the antimicrobial potential of novel actinomycetes has been evaluated by initial screening of six soil samples. Primary and secondary screening was performed against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Candida albicans, Candida tropicalis, Trichophyton rubrum , and other MDR bacterial and fungal test strains, thirteen active isolates were selected for further study. Microbial strains were identified on the basis of growth conditions and other biochemical characters. NCIM
Isolation and screening of microorganisms with potential for biotransformation of terpenic substrates. The objective of the present work was to isolate and select strains with potential to perform the biotransformation of terpenic substrates. Microorganisms obtained from a collection culture and also isolated from a natural source of terpene substrate were tested. Subsequently, 10 strains were selected by their capacity of using these terpenes as sole carbon source in a mineral medium. The biotransformation capacity of these strains was tested and the products obtained were identified by GC-MS.
Environmental pollution with man-made contaminants poses potential risks for humans, animals and plants due to their persistence and toxicity. Contaminants are very different in their chemical structure, show different abundance and stability in the environment and differ in the accessibility for microorganisms. In addition, they can strongly adsorb to soil particles and remain immobilized Boonchan et al. Contaminants have a large extent: from crude oil ingredients as benzene, toluene, ethylbenzene and xylene BTEX , to polycyclic aromatic hydrocarbons PAHs from coal and tar residues, hexadecane from diesel to chemical liquid waste as polychlorinated biphenyls PCB. The removal of these toxic compounds is a challenge and can be very cost intensive.
Xylano-pectino-cellulolytic enzymes are valuable enzymes of the industrial sector. In our earlier study, we have reported a novel and cost effective methodology for the qualitative screening of cellulase-free xylano-pectinolytic microorganisms by replacing the commercial, highly expensive substrates with agricultural residues, but the microorganisms with xylanolytic, pectinolytic, cellulolytic, xylano-pectinolytic, xylano-cellulolytic, pectino-cellulolytic, and xylano-pectino-cellulolytic potential were obtained. The probability of getting the desired combination was low, so efforts were made to further improve this cost effective methodology for obtaining the high yield of the microbes capable of producing desired combination of enzymes. By inclusion of multiple enrichment steps in sequence, using only practically low cost substrates and without any nutrient media till primary screening stage, this improved novel protocol for screening gave only the desired microorganisms with xylano-pectino-cellulolytic activity. Using this rapid, efficient, cost effective, and improved methodology, microbes with required combination of enzymes can be obtained and the probability of getting the desired microorganisms is cent percent. This is the first report presenting the methodology for the isolation of xylano-pectino-cellulolytic positive microorganisms at low cost and consuming less time. Lignocellulose, the most abundant natural biopolymer on earth, is an important source for the production of various industrially useful materials.
Recent Advances in Biotechnology pp Cite as. The unique opportunities provided by recombinant DNA technology are incontestable but for many biotechnological products and processes this option either is unnecessary, inappropriate or unacceptable. The main focus is placed on microbial diversity but reference also is made to other life forms in the context of searching for and discovering novel biochemistry that can be harnessed for biotechnologies. Search strategies can be based upon: 1 screening for desired properties among assemblages of known organisms, or 2 the isolation or detection of novel organisms in the expectation that they will present novel behaviour. Attention also needs to be given to genetic diversity, and to possibilities of accessing non-culturable microorganisms.
sympathy and availability to always get the necessary material for the From the isolation and screening of 98 novel strains, 7 were selected and further characterized. Culture Collection of Industrial Microorganisms, INETI, Lisbon, Portugal.
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